Question No:1
Which is not a vector for r-DNA technology?
[A] Plasmids
[B] Cosmids
[C] Phages
[D] Mosquitoes
Question No:2
Broken ends of two DNA strands are joined by
[A] Exonuclease
[B] Endonuclease
[C] DNA ligase
[D] Gyrases
Question No:3
Electrophoresis and Southern blotting techniques are used in
[A] DNA fingerprinting
[B] Gene synthesis
[C] Gene cloning
[D] All of the above
Question No:4
PCR technique was discovered by
[A] Hamilton Smith
[B] Mullis
[C] E. Southern
[D] Milstein
Question No:5
Genetic engineering means
[A] Gene manipulation
[B] Tissue culture
[C] Utilization of microbes in industries
[D] Somatic hybridixation
Question No:6
Biotechnology helps in synthesizing
[A] New generation antibiotics
[B] New vaccines
[C] MAB
[D] All of the above
Question No:7
Genetic engineering is possible because of the discovery of
[A] Transduction
[B] Transformation
[C] Restriction enzyme and DNA ligase
[D] Electron microscopy
Question No:8
Plasmids are suitable vector for gene cloning because
[A] They are smaller circular DNA
[B] They are linear DNA
[C] They can shuttle between prokaryotes and eukaryotes
[D] None of the above
Question No:9
Bacteria possessing restriction endonuclease enzymes remain
[A] Affected by bacteriophages
[B] Un attacked by bacteriophages
[C] Resistant to drugs and heat
[D] None of the above
Question No:10
Restriction endonuclease cuts
[A] ds-DNA
[B] ss-DNA
[C] Single strand of ds-DNA
[D] Both (b) and (c)
Question No:11
Genes for antibiotic resistance are located on
[A] Bacterial genome
[B] Plasmids
[C] Mesosomes
[D] Plasma membrane
Question No:12
Biotechnology reveals use of
[A] Microorganisms in industrial processes
[B] r-DNA
[C] Engineered bacteria for production of antibiotics and antibodies
[D] All of the above
Question No:13
Manipulation in gene has been made possible because of
[A] Discovery of restriction endonuclease
[B] Development of method for production of r-DNA
[C] Discovery of producing desired genes
[D] All of the above
Question No:14
Tumour producing plasmid transforms
[A] Animals
[B] Plants
[C] Bacteria
[D] Fungi
Question No:15
The specific DNA sequence in which EcoRI cuts is
[A] GATTCG
[B] GAATTC
[C] GTTCAA
[D] TTCCAA
Biotechnology Principles and Processes Mock Test-5
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