Question No:1

Which is not a vector for r-DNA technology?

[A] Plasmids
[B] Cosmids
[C] Phages
[D] Mosquitoes



Question No:2

Broken ends of two DNA strands are joined by

[A] Exonuclease
[B] Endonuclease
[C] DNA ligase
[D] Gyrases



Question No:3

Electrophoresis and Southern blotting techniques are used in

[A] DNA fingerprinting
[B] Gene synthesis
[C] Gene cloning
[D] All of the above



Question No:4

PCR technique was discovered by

[A] Hamilton Smith
[B] Mullis
[C] E. Southern
[D] Milstein



Question No:5

Genetic engineering means

[A] Gene manipulation
[B] Tissue culture
[C] Utilization of microbes in industries
[D] Somatic hybridixation



Question No:6

Biotechnology helps in synthesizing

[A] New generation antibiotics
[B] New vaccines
[C] MAB
[D] All of the above



Question No:7

Genetic engineering is possible because of the discovery of

[A] Transduction
[B] Transformation
[C] Restriction enzyme and DNA ligase
[D] Electron microscopy



Question No:8

Plasmids are suitable vector for gene cloning because

[A] They are smaller circular DNA
[B] They are linear DNA
[C] They can shuttle between prokaryotes and eukaryotes
[D] None of the above



Question No:9

Bacteria possessing restriction endonuclease enzymes remain

[A] Affected by bacteriophages
[B] Un attacked by bacteriophages
[C] Resistant to drugs and heat
[D] None of the above



Question No:10

Restriction endonuclease cuts

[A] ds-DNA
[B] ss-DNA
[C] Single strand of ds-DNA
[D] Both (b) and (c)



Question No:11

Genes for antibiotic resistance are located on

[A] Bacterial genome
[B] Plasmids
[C] Mesosomes
[D] Plasma membrane



Question No:12

Biotechnology reveals use of

[A] Microorganisms in industrial processes
[B] r-DNA
[C] Engineered bacteria for production of antibiotics and antibodies
[D] All of the above



Question No:13

Manipulation in gene has been made possible because of

[A] Discovery of restriction endonuclease
[B] Development of method for production of r-DNA
[C] Discovery of producing desired genes
[D] All of the above



Question No:14

Tumour producing plasmid transforms

[A] Animals
[B] Plants
[C] Bacteria
[D] Fungi



Question No:15

The specific DNA sequence in which EcoRI cuts is

[A] GATTCG
[B] GAATTC
[C] GTTCAA
[D] TTCCAA