Question No:1
Assertion: In EcoR I, the latter R is derived from the genus of bacteria.
Reason: EcoR I is the name of palindromic nucleotide sequences.
[A] If both the assertion and the reason are true and the reason is a correct explanation of the assertion
[B] If both the assertion and reason are true but the reason is not a correct explanation of the assertion
[C] If the assertion is true but the reason is false
[D] If both the assertion and reason are false
Question No:2
Assertion: In r-DNA technology restriction enzymes, those produce sticky ends are Commonly used.
Reason: Sticky ends facilitates the action of enzyme DNA ligase.
[A] If both the assertion and the reason are true and the reason is a correct explanation of the assertion
[B] If both the assertion and reason are true but the reason is not a correct explanation of the assertion
[C] If the assertion is true but the reason is false
[D] If both the assertion and reason are false
Question No:3
Assertion: Cloning vector should have selectable marker.
Reason: Selectable marker, helps in identifying and eleminating non-transformants & selectively permitting the growth of transformants.
[A] If both the assertion and the reason are true and the reason is a correct explanation of the assertion
[B] If both the assertion and reason are true but the reason is not a correct explanation of the assertion
[C] If the assertion is true but the reason is false
[D] If both the assertion and reason are false
Question No:4
Assertion: Retroviruses in animals have ability to transform normal cells into cancerous cells.
Reason: Retroviruses have ability to convert protoncogenes into oncogenes.
[A] If both the assertion and the reason are true and the reason is a correct explanation of the assertion
[B] If both the assertion and reason are true but the reason is not a correct explanation of the assertion
[C] If the assertion is true but the reason is false
[D] If both the assertion and reason are false
Question No:5
Assertion: Micro-injection technique is use to inject r-DNA directly into the nucleus of an animal cell.
Reason: Gene-gun is use to transfer r-DNA into plant cells.
[A] If both the assertion and the reason are true and the reason is a correct explanation of the assertion
[B] If both the assertion and reason are true but the reason is not a correct explanation of the assertion
[C] If the assertion is true but the reason is false
[D] If both the assertion and reason are false
Question No:6
Assertion: In case of PCR the new DNA synthesize on template DNA, one in continuous manner & other in discontinuous manner.
Reason: PCR is an in vivo process.
[A] If both the assertion and the reason are true and the reason is a correct explanation of the assertion
[B] If both the assertion and reason are true but the reason is not a correct explanation of the assertion
[C] If the assertion is true but the reason is false
[D] If both the assertion and reason are false
Question No:7
Assertion: In bioreactors the transforming cells are maintained hi their physiologically most active log / exponential phase.
Reason: This type culturing methods produces a larger biomass using to higher yields of desired protein.
[A] If both the assertion and the reason are true and the reason is a correct explanation of the assertion
[B] If both the assertion and reason are true but the reason is not a correct explanation of the assertion
[C] If the assertion is true but the reason is false
[D] If both the assertion and reason are false
Question No:8
Which one of the following is now being commercially produced by biotechnological procedures?
[A] Nicotine
[B] Morphine
[C] Quinine
[D] Insulin
Question No:9
Which of the following are used in gene cloning?
[A] Nucleosides
[B] Lomasomes
[C] Mesosomes
[D] Plasmids
Question No:10
In genetic engineering, a DNA segment (gene) of interest is transferred to the host cell through a vector. Consider the following four agents (i – iv) in this regard and select the correct option about which one or more of these can be used as a vector/ vectors (A) A Bacterium
(B) Plasmid
(C) Plasmodium
(D) Bacteriophage
[A] (A) , (B) & (D) only
[B] (A) only
[C] (A)&(C)only
[D] (B) & (D) only
Question No:11
Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?
[A] 5‘-CGTTCG-3‘3‘-ATGGTA-5‘
[B] 5‘-GATATG-3‘ 3‘-CTACTA-5‘
[C] 5‘-GAATTC-3‘ 3‘-CTTAAG-5‘
[D] 5‘-CACGTA-3‘ 3‘-CTCAGT- 5‘
Question No:12
Restriction endonucleases are enzymes which:
[A] Make cuts at specific positions within the DNA molecule
[B] Recognize a specific nucleotide sequence for binding of DNA ligase
[C] Restrict the action of the enzyme DNA polymerase
[D] Remove nucleotides from the ends of the DNA molecule
Question No:13
Stirred-tank bioreactors have been designed for
[A] Addition of preservatives of the product
[B] Purification of the product
[C] Ensuring anaerobic conditions in the culture vessel
[D] Availability of oxygen throughout the process
Biotechnology Principles and Processes Mock Test-8
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