Question No:1

Assertion: In EcoR I, the latter R is derived from the genus of bacteria.
Reason: EcoR I is the name of palindromic nucleotide sequences.

[A] If both the assertion and the reason are true and the reason is a correct explanation of the assertion
[B] If both the assertion and reason are true but the reason is not a correct explanation of the assertion
[C] If the assertion is true but the reason is false
[D] If both the assertion and reason are false



Question No:2

Assertion: In r-DNA technology restriction enzymes, those produce sticky ends are Commonly used.
Reason: Sticky ends facilitates the action of enzyme DNA ligase.

[A] If both the assertion and the reason are true and the reason is a correct explanation of the assertion
[B] If both the assertion and reason are true but the reason is not a correct explanation of the assertion
[C] If the assertion is true but the reason is false
[D] If both the assertion and reason are false



Question No:3

Assertion: Cloning vector should have selectable marker.
Reason: Selectable marker, helps in identifying and eleminating non-transformants & selectively permitting the growth of transformants.

[A] If both the assertion and the reason are true and the reason is a correct explanation of the assertion
[B] If both the assertion and reason are true but the reason is not a correct explanation of the assertion
[C] If the assertion is true but the reason is false
[D] If both the assertion and reason are false



Question No:4

Assertion: Retroviruses in animals have ability to transform normal cells into cancerous cells.
Reason: Retroviruses have ability to convert protoncogenes into oncogenes.

[A] If both the assertion and the reason are true and the reason is a correct explanation of the assertion
[B] If both the assertion and reason are true but the reason is not a correct explanation of the assertion
[C] If the assertion is true but the reason is false
[D] If both the assertion and reason are false



Question No:5

Assertion: Micro-injection technique is use to inject r-DNA directly into the nucleus of an animal cell.
Reason: Gene-gun is use to transfer r-DNA into plant cells.

[A] If both the assertion and the reason are true and the reason is a correct explanation of the assertion
[B] If both the assertion and reason are true but the reason is not a correct explanation of the assertion
[C] If the assertion is true but the reason is false
[D] If both the assertion and reason are false



Question No:6

Assertion: In case of PCR the new DNA synthesize on template DNA, one in continuous manner & other in discontinuous manner.
Reason: PCR is an in vivo process.

[A] If both the assertion and the reason are true and the reason is a correct explanation of the assertion
[B] If both the assertion and reason are true but the reason is not a correct explanation of the assertion
[C] If the assertion is true but the reason is false
[D] If both the assertion and reason are false



Question No:7

Assertion: In bioreactors the transforming cells are maintained hi their physiologically most active log / exponential phase.
Reason: This type culturing methods produces a larger biomass using to higher yields of desired protein.

[A] If both the assertion and the reason are true and the reason is a correct explanation of the assertion
[B] If both the assertion and reason are true but the reason is not a correct explanation of the assertion
[C] If the assertion is true but the reason is false
[D] If both the assertion and reason are false



Question No:8

Which one of the following is now being commercially produced by biotechnological procedures?

[A] Nicotine
[B] Morphine
[C] Quinine
[D] Insulin



Question No:9

Which of the following are used in gene cloning?

[A] Nucleosides
[B] Lomasomes
[C] Mesosomes
[D] Plasmids



Question No:10

In genetic engineering, a DNA segment (gene) of interest is transferred to the host cell through a vector. Consider the following four agents (i – iv) in this regard and select the correct option about which one or more of these can be used as a vector/ vectors (A) A Bacterium
(B) Plasmid
(C) Plasmodium
(D) Bacteriophage

[A] (A) , (B) & (D) only
[B] (A) only
[C] (A)&(C)only
[D] (B) & (D) only



Question No:11

Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?

[A] 5^‘-CGTTCG-3^‘3^‘-ATGGTA-5^‘
[B] 5^‘-GATATG-3^‘ 3^‘-CTACTA-5^‘
[C] 5^‘-GAATTC-3^‘ 3^‘-CTTAAG-5^‘
[D] 5^‘-CACGTA-3^‘ 3^‘-CTCAGT- 5^‘



Question No:12

Restriction endonucleases are enzymes which:

[A] Make cuts at specific positions within the DNA molecule
[B] Recognize a specific nucleotide sequence for binding of DNA ligase
[C] Restrict the action of the enzyme DNA polymerase
[D] Remove nucleotides from the ends of the DNA molecule



Question No:13

Stirred-tank bioreactors have been designed for

[A] Addition of preservatives of the product
[B] Purification of the product
[C] Ensuring anaerobic conditions in the culture vessel
[D] Availability of oxygen throughout the process