Question No:1

Which method is suitable for transferring an alien DNA into a plant cell?

[A] CaCl₂
[B] Biolistics or gene gun
[C] Micro-infection
[D] Heat shock



Question No:2

Arrange the following process in the sequence used in r-DNA technology.
(1) Isolation of DNA
(2) Fragmentation of DNA by RE
(3) Isolation of desired DNA fragment
(4) Ligation of DNA fragment into vector
(5) Transferring r-DNA into host
(6) Culturing host cells in a medium at large scale
(7) Extraction of the desired product

[A] 1,2,3,4,5,6,7
[B] 1,2,3,4,6,5,7
[C] 2, 1, 3, 4, 5, 6, 7
[D] 1, 2, 3, 5, 4, 6, 7



Question No:3

In majority of organism genetic material is

[A] DNA
[B] RNA
[C] m-RNA
[D] Protein



Question No:4

In order to extract DNA we use the following enzyme for the following organism, which matching is correct?

[A] Plant cell-Cellulose
[B] Fungus-Chitinase
[C] Bacteria-Lysozyme
[D] All are correct



Question No:5

In isolation of genetic material (DNA) from nucleus, the RNA can be removed by ribonuclease and protein by protease. Purified DNA then precipitates after the addition of ______

[A] CaCl₂
[B] ether
[C] chilled ethanol
[D] acetic acid



Question No:6

Which of the following is used to check progression of restriction enzyme digestion?

[A] PCR
[B] ROP
[C] AGE
[D] All



Question No:7

PCR stands for

[A] Polymer chain reaction
[B] Polymerase chain reactor
[C] Polymerase chain reaction
[D] Polymerisation, combination, Recognition



Question No:8

PCR includes

[A] Denaturation
[B] Annealing
[C] Extension
[D] All of these



Question No:9

30 cycles of PCR amplified DNA approx how many times

[A] 1 billion times
[B] 1 million times
[C] 100 times
[D] 1000 times



Question No:10

If any protein encoding gene is expressed in a ______ host, it is called a ______ protein.

[A] homologus host, recombinant
[B] heterologus host, recombinant
[C] heterologus host, non-recombinant
[D] homologus host, non-recombinant



Question No:11

What is the maximum volume of culture that can be processed in bioreactors?

[A] 10-100 liters
[B] 100-1000 liters
[C] 1-10 liters
[D] 1000-1,00,000 liters



Question No:12

The host cultured in a continuous culture system where inside the used medium is drained out from one side while fresh medium is added from the other to maintain cells in their physiologically ______ phase.

[A] lag
[B] log
[C] stationary
[D] declining



Question No:13

Bioreactors have

[A] Foam control system, temperature control system
[B] Oxygen delivery system
[C] pH control system
[D] All of the above



Question No:14

Following will be done with product formed by r-DNA technology?

[A] Product has to be formulated with suitable preservation.
[B] Formulation has to undergo clinical trials.
[C] Strict quality control testing is done.
[D] All of the above



Question No:15

r-DNA is a

[A] Chimeric DNA
[B] Hybrid DNA-RNA
[C] Recombinant of vector DNA and desired gene/s
[D] Both (a) and (c)