Question No:1
Which method is suitable for transferring an alien DNA into a plant cell?
[A] CaCl2
[B] Biolistics or gene gun
[C] Micro-infection
[D] Heat shock
Question No:2
Arrange the following process in the sequence used in r-DNA technology.
(1) Isolation of DNA
(2) Fragmentation of DNA by RE
(3) Isolation of desired DNA fragment
(4) Ligation of DNA fragment into vector
(5) Transferring r-DNA into host
(6) Culturing host cells in a medium at large scale
(7) Extraction of the desired product
[A] 1,2,3,4,5,6,7
[B] 1,2,3,4,6,5,7
[C] 2, 1, 3, 4, 5, 6, 7
[D] 1, 2, 3, 5, 4, 6, 7
Question No:3
In majority of organism genetic material is
[A] DNA
[B] RNA
[C] m-RNA
[D] Protein
Question No:4
In order to extract DNA we use the following enzyme for the following organism, which matching is correct?
[A] Plant cell-Cellulose
[B] Fungus-Chitinase
[C] Bacteria-Lysozyme
[D] All are correct
Question No:5
In isolation of genetic material (DNA) from nucleus, the RNA can be removed by ribonuclease and protein by protease. Purified DNA then precipitates after the addition of ______
[A] CaCl2
[B] ether
[C] chilled ethanol
[D] acetic acid
Question No:6
Which of the following is used to check progression of restriction enzyme digestion?
[A] PCR
[B] ROP
[C] AGE
[D] All
Question No:7
PCR stands for
[A] Polymer chain reaction
[B] Polymerase chain reactor
[C] Polymerase chain reaction
[D] Polymerisation, combination, Recognition
Question No:8
PCR includes
[A] Denaturation
[B] Annealing
[C] Extension
[D] All of these
Question No:9
30 cycles of PCR amplified DNA approx how many times
[A] 1 billion times
[B] 1 million times
[C] 100 times
[D] 1000 times
Question No:10
If any protein encoding gene is expressed in a ______ host, it is called a ______ protein.
[A] homologus host, recombinant
[B] heterologus host, recombinant
[C] heterologus host, non-recombinant
[D] homologus host, non-recombinant
Question No:11
What is the maximum volume of culture that can be processed in bioreactors?
[A] 10-100 liters
[B] 100-1000 liters
[C] 1-10 liters
[D] 1000-1,00,000 liters
Question No:12
The host cultured in a continuous culture system where inside the used medium is drained out from one side while fresh medium is added from the other to maintain cells in their physiologically ______ phase.
[A] lag
[B] log
[C] stationary
[D] declining
Question No:13
Bioreactors have
[A] Foam control system, temperature control system
[B] Oxygen delivery system
[C] pH control system
[D] All of the above
Question No:14
Following will be done with product formed by r-DNA technology?
[A] Product has to be formulated with suitable preservation.
[B] Formulation has to undergo clinical trials.
[C] Strict quality control testing is done.
[D] All of the above
Question No:15
r-DNA is a
[A] Chimeric DNA
[B] Hybrid DNA-RNA
[C] Recombinant of vector DNA and desired gene/s
[D] Both (a) and (c)
Biotechnology Principles and Processes Mock Test-3
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