Question No:1

PCR and Restriction Fragment Length Polymorphism are the methods for:

[A] Study of enzymes
[B] Genetic transformation
[C] DNA sequencing
[D] Genetic Fingerprinting



Question No:2

Which one of the following is not a part of a transcription unit in DNA?

[A] The inducer
[B] A terminator
[C] A promoter
[D] The structural gene



Question No:3

A single strand of nucleic acid tagged with a radioactive molecule is called:

[A] Vector
[B] Selectable market
[C] Plasmid
[D] Probe



Question No:4

If one strand of DNA has the nitrogenous base sequence as ATCTG, what would be the complementary RNA strand sequence?

[A] TTAGU
[B] UAGAC
[C] AACTG
[D] ATCGU



Question No:5

Removal of introns and joining of exons in a defined order during transcription is called:

[A] Looping
[B] Inducing
[C] Slicing
[D] Splicing



Question No:6

Which enzyme/s will be produced in a cell in which there is a nonsense mutation in the lac Y gene?

[A] beta-galactosidase
[B] Lactose permease
[C] Transacetylase
[D] Lactose permease and transacetylase



Question No:7

Which one of the following is wrongly matched?

[A] Transcription – Writing information from DNA to t – RNA
[B] Translation – Using information in m-RNA to make protein
[C] Represser protein — Binds to operator to stop enzyme synthesis
[D] Operon — Structural genes, operator and promoter



Question No:8

Transformation was discovered by:

[A] Meselson and Stahl
[B] Hershey and Chase
[C] Griffith
[D] Watson and Crick



Question No:9

An analysis of chromosomal DNA using the Southern hybridization technique does not use

[A] Electrophoresis
[B] Blotting
[C] Autoradiography
[D] PCR



Question No:10

Select the correct option: (A: Direction RNA synthesis, B: Direction of reading of the template DNA strand)

[A] 5 ^’——-3^’ 3^’——5^’
[B] 3^’——-5^’ 5^’——3^’
[C] 5^’——-3^’ 5^’——3^’
[D] 3^’——-5^’ 3^’——5^’



Question No:11

Commonly used vectors for human genome sequencing are:

[A] T-DNA
[B] BA C and YAC
[C] Expression Vectors
[D] T/Cloning Vectors



Question No:12

Gene regulation governing lactose operon of E. coli that involves the lad gene product is:

[A] Positive and inducible because it can be induced by lactose
[B] Negative and inducible because represser protein prevents transcription
[C] Negative and repressible because represser protein prevents transcription
[D] Feedback inhibition because excess of p galactosidase can switch off transcription.



Question No:13

In sea urchin DNA, which is double stranded, 17% of the bases were shown to be cytosine. The percentages of the other three bases expected to be present in this DNA are :

[A] G 34%, A 24.5%, T 24.5%
[B] G 17%, A 16.5%, T 32.5%
[C] G 17%, A 33%, T 33%
[D] G 8.5%, A 50%, T 24.5%



Question No:14

Which one of the following is not applicable to RNA?

[A] 5^’ phosphoryl and 3′ hydroxyl ends
[B] Heterocyclic nitrogeneous bases
[C] Chargaff’s rule
[D] Complementary base pairing



Question No:15

Identify the correct order of organization of genetic material from largest to smallest:

[A] Genome, chromosome, nucleotide, gene
[B] Genome, chromosome, gene, nucleotide
[C] Chromosome, genome, nucleotide, gene
[D] Chromosome, gene, genome, nucleotide



Question No:16

Balbiani rings are sites of

[A] Nucleotide synthesis
[B] Polysaccharide synthesis
[C] RNA and protein synthesis
[D] Lipid synthesis



Question No:17

Satellite DNA is important because it:

[A] Shows high degree of polymorphism in population and also the same degree of polymorphism in an individual, which is heritable from parents to children
[B] Does not code for proteins and is same in all members of the population
[C] Codes for enzymes needed for DNA repletion
[D] Codes for proteins needed hi cell cycle.