Restriction enzyme breaks
[A] Glycosidic linkage
[C] Sugar-phosphate linkage
[D] All of the above
Sequence recognize by Eco RI is
[A] 5‘-GAATTC-3‘ 3‘-CTTAAG-5‘
[B] 5‘-CCAATG-3‘ 3‘-GAATCC-5
[C] 5‘-GATACC-3‘ 3‘-CCTAAG-5‘
[D] 5‘-CATTAG-3‘ 3‘-GATAAC-5‘
EcoRI cut palindrome sequence which produces overhanging stretches called sticky ends on each strand. These are named sticky because
[A] They can combine with any DNA.
[B] They form hydrogen bonds with their complementary cut counterpart.
[C] They facilitate the action of the enzyme DNA ligase.
[D] Both (b) and (c)
The extraction of DNA from a gel piece is known as
The fragmented DNA can be visualized by staining DNA with
[B] Ethidium bromide
[C] Ethylene bromide
For cloning of foreign DNA it is attached with origin of vector which control
[B] Copy number of replicating DNA
[C] Both (a) and (b)
Find the true statement.
[A] Ori means origin of transcription.
[B] Some bacterial cell may have copy number of plasmid vary from 15—100.
[C] Vector should have many recognition sites for commonly used restriction enzymes so that alien DNA can attach to any one of the sites easily.
[D] tetR gene in pBR322 can be cleared by Pvu I and Pst I.
Following antibiotic resistance genes are used as selectable marker for E. coli
[D] All of these
If alien DNA in introduced using Sal I in pBR322 then transformant will grow on
Select the incorrect matching
[A] pBR322 – E. coli cloning vector
[B] Eco R I, Cla – Restriction enzyme I, Hind IE
[C] ROP — Protein involved in the replication of the plasmid
[D] PCR – Technique in which multiple copies of the gene (or DNA) ___________of interest hi synthesized in vitro _______
Select the incorrect statement.
[A] Selection of recombinants due to inactivation of antibiotic is a cumbersome procedure.
[B] Insertional inactivation of β-galactosidase leads to colorless colonies.
[C] Insertional inactivation of β-glactosidase leads to blue color colonies.
[D] In insertional inactivation, the r-DNA is inserted within the coding sequence of an enzyme β-glactosidase.
Which vector is use to deliver gene in animal cell?
[B] Disarmed retroviruses
[D] E. coli
To make bacterium competent (Transformation with recombinant DNA) we use
[A] Specific concentration of Ca2+ ion
[B] Heat shock (42℃)
By which method is rDNA directly placed in nucleus of animal cell?
[A] Gene gun (biolistics)
[B] Heat shock
Way to introduce alien DNA into host cell includes
[A] Disarmed pathogens
[B] Biolistics or gene gun
[D] All of the above