Question No:1

In the PCR technology, the DNA segment is replicated over a billion times. This repeated replication is catalyzed by the enzyme

[A] DNA polymerase
[B] Taq polymerase
[C] DNA dependent RNA polymerase
[D] Primase



Question No:2

An antibiotic resistance gene in a vector usually helps in the selection of

[A] Competent cells
[B] Transformed cells
[C] Recombinant cells
[D] None of the above



Question No:3

Significance of ‘heat shock’ method in bacterial transformation is to facilitate

[A] binding of DNA to the cell wall
[B] uptake of DNA through membrane transport proteins
[C] uptake of DNA through transient pores in the bacterial cell wall
[D] expression of antibiotic resistance gene



Question No:4

The role of DNA ligase in the construction of a recombinant DNA molecule is

[A] Formation of phosphodiester bond between two DNA fragments
[B] Formation of hydrogen bonds between sticky ends of DNA fragments
[C] Ligation of all purine and pyrimidine bases
[D] None of the above



Question No:5

Which of the following bacteria is not a source of restriction endonuclease?

[A] Haemophilus influenzae
[B] Escherichia coli
[C] Agrobacterium tumefaciens
[D] Bacillius amyloli



Question No:6

Which of the following steps are catalysed by Taq polymerase in a PCR reaction?

[A] Denaruration of template DNA
[B] Annealing of primers to template DNA
[C] Extension of primer end on the template DNA
[D] All of the above



Question No:7

A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be

[A] human gene may have intron which bacteria cannot process.
[B] amino acid codons for humans and bacteria are different.
[C] human protein is formed but degraded by bacteria.
[D] all of the above



Question No:8

Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?

[A] Laboratory flask of largest capacity
[B] A stirred-tank bioreactor without in-lets and outlets
[C] A continuous culture system
[D] Any of the above



Question No:9

Who among the following was awarded the Nobel Prize for the development of PCR technique?

[A] Herbert Boyer
[B] Hargovind Khurana
[C] Kary Mullis
[D] Arthur Kornberg



Question No:10

Which of the following statements does not hold true for restriction enzyme?

[A] It recognises a palindromic nucleotide sequence.
[B] It is an endonuclease.
[C] It is isolated from viruses.
[D] It produces the same kind of sticky ends in different DNA molecules.



Question No:11

Assertion: Biotechnology deals with techniques of using live organism or enzymes from organisms to produce products & processes useful of humans.
Reason: In vitro fertilization is a part of biotechnology.

[A] If both the assertion and the reason are true and the reason is a correct explanation of the assertion
[B] If both the assertion and reason are true but the reason is not a correct explanation of the assertion
[C] If the assertion is true but the reason is false
[D] If both the assertion and reason are false



Question No:12

Assertion: r-DNA technology is superior over hybridisation.
Reason: r-DNA technology allow us to isolate & introduce only one or a set of desirable gene without introducing undesirable genes into the target organism.

[A] If both the assertion and the reason are true and the reason is a correct explanation of the assertion
[B] If both the assertion and reason are true but the reason is not a correct explanation of the assertion
[C] If the assertion is true but the reason is false
[D] If both the assertion and reason are false



Question No:13

Assertion: Restriction endonuclease restrict the growth of bacteriophage in bacteria.
Reason: Restriction endonuclease add methyl groups to bacterial DNA.

[A] If both the assertion and the reason are true and the reason is a correct explanation of the assertion
[B] If both the assertion and reason are true but the reason is not a correct explanation of the assertion
[C] If the assertion is true but the reason is false
[D] If both the assertion and reason are false