Question No:1

Which one of the following techniques made it possible to genetically engineer living organisms?

[A] Recombinant DNA techniques
[B] X-ray diffraction
[C] Heavier isotope labelling
[D] Hybridization



Question No:2

Given below is a sample of portion of DNA strand giving the base sequence on the opposite strands. What is so, special shown in it?
5^‘_GAATTC_3^‘
3^‘_CTTAAG_5^‘

[A] Deletion mutation
[B] Start codon at the 5^‘ end
[C] Palindromic sequence of base pairs
[D] Replication completed



Question No:3

There is a restriction endonuclease called Eco RI. What does ‘co’ part in it stand for?

[A] Coelom
[B] Coenzyme
[C] Coli
[D] Colon



Question No:4

Agarose extracted from sea weeds finds use in

[A] Tissue culture
[B] PCR
[C] Gel electrophoresis
[D] Spectrophotometry



Question No:5

Maximum number of existing transgenic animals is of.

[A] Mice
[B] Cow
[C] Pig
[D] Fish



Question No:6

In genetic engineering, the antibiotics are used:

[A] To select healthy vectors
[B] As sequences from where replication starts
[C] To keep the cultures free of infection
[D] As selectable markers



Question No:7

Which one of the following represents a palindromic sequence in?

[A] 5^‘-CCAATG-3^‘
[B] 5^‘-CATTAG-3^‘ 3^‘-GAATCC-5^‘ 3^‘-GATAAC-5^‘
[C] 5^‘-GATACC-3^‘
[D] 5^‘-GAATTC-3^‘ 3^‘-CCTAAG-5^‘ 3^‘-CTTAAG-5^‘



Question No:8

Biolistics (gene-gun) is suitable for:

[A] Transformation of plant cells
[B] Constructing recombinant DNA by joining with vectors
[C] DNA finger printing
[D] Disarming pathogen vectors



Question No:9

Which one is a true statement regarding DNA polymerase used in PCR?

[A] It is used to ligate introduced DNA in recipient cells
[B] It serves as a selectable marker
[C] It is isolated from a virus
[D] It remains active at high temperature



Question No:10

For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of:

[A] Silver or Platinum
[B] Platinum or Zinc
[C] Silicon or Platinum
[D] Gold or Tungsten



Question No:11

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by:

[A] Centrifugation
[B] Polymerase chain reaction
[C] Electrophoresis
[D] Restriction mapping



Question No:12

Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?

[A] Bacteria – Lysozyme
[B] Plant cells – Cellulase
[C] Algae – Methylase
[D] Fungi – Chitinase



Question No:13

Which vector can clone only a small fragment of DNA?

[A] Bacteria artificial chromosome
[B] Yeast artificial chromosome
[C] Plasmid
[D] Cosmid



Question No:14

The cutting of DNA at specific locations because possible with the discovery of:

[A] Probes
[B] Selectable markers
[C] Ligases
[D] Restriction enzymes



Question No:15

The DNA molecule to which the gene of interest is integrated for cloning is called:

[A] Vector
[B] Template
[C] Carrier
[D] Transformer