Which one of the following techniques made it possible to genetically engineer living organisms?
[A] Recombinant DNA techniques
[B] X-ray diffraction
[C] Heavier isotope labelling
Given below is a sample of portion of DNA strand giving the base sequence on the opposite strands. What is so, special shown in it?
[A] Deletion mutation
[B] Start codon at the 5‘ end
[C] Palindromic sequence of base pairs
[D] Replication completed
There is a restriction endonuclease called Eco RI. What does ‘co’ part in it stand for?
Agarose extracted from sea weeds finds use in
[A] Tissue culture
[C] Gel electrophoresis
Maximum number of existing transgenic animals is of.
In genetic engineering, the antibiotics are used:
[A] To select healthy vectors
[B] As sequences from where replication starts
[C] To keep the cultures free of infection
[D] As selectable markers
Which one of the following represents a palindromic sequence in?
[B] 5‘-CATTAG-3‘ 3‘-GAATCC-5‘ 3‘-GATAAC-5‘
[D] 5‘-GAATTC-3‘ 3‘-CCTAAG-5‘ 3‘-CTTAAG-5‘
Biolistics (gene-gun) is suitable for:
[A] Transformation of plant cells
[B] Constructing recombinant DNA by joining with vectors
[C] DNA finger printing
[D] Disarming pathogen vectors
Which one is a true statement regarding DNA polymerase used in PCR?
[A] It is used to ligate introduced DNA in recipient cells
[B] It serves as a selectable marker
[C] It is isolated from a virus
[D] It remains active at high temperature
For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of:
[A] Silver or Platinum
[B] Platinum or Zinc
[C] Silicon or Platinum
[D] Gold or Tungsten
DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by:
[B] Polymerase chain reaction
[D] Restriction mapping
Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?
[A] Bacteria – Lysozyme
[B] Plant cells – Cellulase
[C] Algae – Methylase
[D] Fungi – Chitinase
Which vector can clone only a small fragment of DNA?
[A] Bacteria artificial chromosome
[B] Yeast artificial chromosome
The cutting of DNA at specific locations because possible with the discovery of:
[B] Selectable markers
[D] Restriction enzymes
The DNA molecule to which the gene of interest is integrated for cloning is called: